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In contrast to random primers, oligo (dT) primers will not be suitable for degraded samples but are excellent for developing cDNA libraries and recommended for use with reverse transcriptases (RTs) as Superscript IV RT.
In contrast to oligo(dT) primers, these primers are certainly not template particular and so are ideal for DNA synthesis applying Klenow fragments with DNA templates or for cDNA synthesis applying reverse transcriptase with mRNA templates.
When dealing with primers, make sure you perform inside of a well-ventilated place free of open flames or sparks. Hold primers far from too much warmth and electrical gear. Use protection Eyeglasses and gloves to safeguard towards accidental discharge.
These items include things like random primers and oligo (dT) primers for to start with strand cDNA synthesis, gene precise primers for sequencing, ahead and reverse sequencing primers and kits, as well as the MegaPlex RT Primers and Megaplex primer swimming pools designed for evaluating expression profiles.
an indication that shows the phrases “Risk — Fire Hazard/Risque d’incendie” in letters at the least ten cm high and that prohibits using tobacco applying letters, or even a symbol, at least ten cm high have to be posted on the storage unit inside of a Plainly seen spot.
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In distinction to random primers, oligo (dT) primers will not be suitable for degraded samples but are superb for setting up cDNA libraries and encouraged for use with reverse transcriptases (RTs) as Superscript IV RT.
I can inform you that some manufacturers that aren’t touted as “match” are already steady. That every one arises from expertise: try diverse primers, just regard the necessity to to begin with decrease the load for each examination.
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